NucleaRDB: Extraction of mutation data from the literature

2005, NucleaRDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation C313S was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation C313S in PPAT_HUMAN

Point mutation:	C313S
Cited point mutation:	Cys313Ser,Cys313
Domain:	LBD HELIX 3
General numbering (NucleaRDB):	335
Protein:	PPAT_HUMAN (PPARG,NR1C)	Swiss-Prot Cross-reference table Family page
Protein isoforms	2
Other point mutations / same protein	List of mutations in PPAT_HUMAN
Family alignments	1C3 PPAR gamma 1C Peroxisome proliferator activated (PPAR) 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR
Other point mutations / same position	Position 218 in 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR family
Reference:	T0070907, a selective ligand for peroxisome proliferator-activated receptor gamma, functions as an antagonist of biochemical and cellular activities. Lee G, Elwood F, McNally J, Weiszmann J, Lindstrom M, Amaral K, Nakamura M, Miao S, Cao P, Learned RM, Chen JL, Li Y J Biol Chem 2002 May 31;277(22):19649-57.	Medline
Other point mutations / same article	List
Text source	HTML and PDF full texts
Validation status	True positive

Relevant sentences:

Cys313

T0070907 Covalently Modifies PPAR gamma on Cys313-- To understand the mechanism by which T0070907 antagonizes PPAR gamma function , its binding properties were first examined
The mass difference between the y3- and y4-ions (m / z 344.1) identified Cys313 as the site of modification by T0070907
T0070907 covalently binds to PPAR gamma at Cys313
Cys313 is indicated by *
To confirm the importance of Cys313 in T0070907 binding to PPAR gamma , a mutant PPAR gamma was constructed in which Cys313 was converted to a serine residue , and the corresponding recombinant GST-PPAR gamma LBD (C313S) fusion protein was expressed and purified
Thus , Cys313 is necessary for the binding of T0070907 to PPAR gamma
Proteolytic mapping indicated that T0070907 irreversibly modifies PPAR gamma on Cys313 in helix 3 of the LBD , a residue that is conserved in all three PPAR subtypes
T0070907 Covalently Modifies PPAR on Cys313--To understand the mechanism by which T0070907 antagonizes PPAR function , its binding properties were first examined
To confirm the importance of Cys313 in T0070907 binding to PPAR , a mutant PPAR was constructed in which Cys313 was converted to a serine residue , and the corresponding recombinant GST-PPAR LBD (C313S) fusion protein was expressed and purified
Thus , Cys313 is necessary for the binding of T0070907 to PPAR
T0070907 covalently binds to PPAR at Cys313
Proteolytic mapping indicated that T0070907 irreversibly modifies PPAR on Cys313 in helix -->of the LBD , a residue that is conserved in all three PPAR subtypes

C313S

B , wt or C313S mutant PPAR gamma proteins were incubated with [3H]T0070907 and subsequently separated on an SDS-polyacrylamide gel , which was then subjected to autoradiography
To confirm the importance of Cys313 in T0070907 binding to PPAR gamma , a mutant PPAR gamma was constructed in which Cys313 was converted to a serine residue , and the corresponding recombinant GST-PPAR gamma LBD (C313S) fusion protein was expressed and purified
[3H]T0070907 was first incubated with either wild (wt) type PPAR gamma or the C313S mutant as described , and the reaction mixtures were separated by SDS-PAGE
As shown in Fig. 3 B , [3H]T0070907 was only able to modify the wild type protein (upper panel , autoradiograph) , although equal amounts of wild type and C313S mutant PPAR gamma were added to each reaction (lower panel , Coomassie staining)
To confirm the importance of Cys313 in T0070907 binding to PPAR , a mutant PPAR was constructed in which Cys313 was converted to a serine residue , and the corresponding recombinant GST-PPAR LBD (C313S) fusion protein was expressed and purified
[3H]T0070907 was first incubated with either wild (wt) type PPAR or the C313S mutant as described , and the reaction mixtures were separated by SDS-PAGE
As shown in Fig. 3B , [3H]T0070907 was only able to modify the wild type protein (upper panel , autoradiograph) , although equal amounts of wild type and C313S mutant PPAR were added to each reaction (lower panel , Coomassie staining)
B , wt or C313S mutant PPAR proteins were incubated with [3H]T0070907 and subsequently separated on an SDS-polyacrylamide gel , which was then subjected to autoradiography

F.Horn (nucleardbcmbi.kun.nl), 21-Apr-2005