NucleaRDB: Extraction of mutation data from the literature

2005, NucleaRDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation L316A was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation L316A in NR1H3_HUMAN

Point mutation:	L316A
Domain:	Loop 5-6
General numbering (NucleaRDB):	-
Protein:	NR1H3_HUMAN (NR1H3,LXR)	Swiss-Prot Cross-reference table Family page
Protein isoforms	2
Other point mutations / same protein	List of mutations in NR1H3_HUMAN
Family alignments	1H3 Oxysterol LXR alpha 1H Ecdysone-like (ECR,LXR,FXR) 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR
Other point mutations / same position	Position 418 in 1H Ecdysone-like (ECR,LXR,FXR) family Position 277 in 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR family
Reference:	Molecular determinants of LXRalpha agonism. Wang M, Thomas J, Burris TP, Schkeryantz J, Michael LF J Mol Graph Model 2003 Nov;22(2):173-81.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

L316A

The sense strand oligonucleotide sequences are as follows: F254A GGCCCGTCAGCAGCGCGCTGCCCACTTCACTGAGC F257A GCAGCGCTTTGCCCACGCCACTGAGCTGGCCATC M298A GACCTCTGCGATCGAGGTGGCGCTTCTGGAGACATCTCG L299A GCGATCGAGGTGATGGCTCTGGAGACATCTCGGAGG E301A CGAGGTGATGCTTCTGGCGACATCTCGGAGGTACAACC T302A GCTTCTGGAGGCATCTCGGAGGTACAACC R305L GCTTCTGGAGACATCTCGGCTGTACAACCCTGGG F315A CACTTCCTCAAGGATGCCAGTTATAACCGGGAAG L316A GGAGTGAGAGTATCACCTTCGCCAAGGATTTCAGTTATAACCGGG L331A GGAAGACTTTGCCAAAGCAGGGGCGCAAGTGGAATTCATCAA F335A GCAGGGCTGCAAGTGGAAGCCATCAACCCCATCTTCG H421A GGACCCTGAGCAGCGTCGCCTCAGAGCAAGTGTTTGC Q424A GCAGCGTCCACTCAGAGGCAGTGTTTGCACTGCGTCTGC L439A GCTCCCACCGCTGGCCTCTGAGATCTGGGATGTGC I442A GCTGCACATCCCAGGCCTCAGAGAGCAGCGGTGGG W443Y CACCGCTGCTCTCTGAGATCTACGATGTGCACGAATG Mutagenesis was confirmed by sequence analysis
Mutations W443Y , L299A and L316A revealed ligand-specific interaction of these residues in the transcriptional assay
The L316A mutation caused a surprising increase in T0901317 efficacy and a moderate decrease in GW3965- and 22RHC-induced transcription
It may be possible , though , that T0901317 increases the efficiency of co-factor recruitment in the context of the L316A mutant

F.Horn (nucleardbcmbi.kun.nl), 21-Apr-2005