NucleaRDB: Extraction of mutation data from the literature

2005, NucleaRDB.

This data was extracted from Medline abstracts and full texts (when available) in an automated manner.

The table below describes the selected point mutation and provides links to other documents. The sentence(s) where the point mutation E542A was found are listed after the table.

The mutated residues are also indicated in the family sequence alignments and hyperlinked to the corresponding mutation pages.

Point mutation E542A in RORA_HUMAN

Point mutation:	E542A
Cited point mutation:	E509A
Domain:	C-term
General numbering (NucleaRDB):	-
Protein:	RORA_HUMAN (RORA,NR1F1, RZR)	Swiss-Prot Cross-reference table Family page
Protein isoforms	4
Other point mutations / same protein	List of mutations in RORA_HUMAN
Family alignments	1F1 ROR alpha 1F RAR-related orphan receptor (ROR,HR3) 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR
Other point mutations / same position	Position 403 in 1 Thyroid hormone like (TR,RAR,ROR,PPAR,VDR family
Reference:	Crystal structure of the human RORalpha Ligand binding domain in complex with cholesterol sulfate at 2.2 A. Kallen J, Schlaeppi JM, Bitsch F, Delhon I, Fournier B J Biol Chem 2004 Apr 2;279(14):14033-8. Epub 2004 Jan 13.	Medline
Other point mutations / same article	List
Text source	HTML full text
Validation status	Not yet checked

Relevant sentences:

E509A

The ROR alpha mutants C228Q , A330Q , A371Q , C323L , and K339A , E509A were generated using a QuikChange XL site-directed mutagenesis kit from Stratagene (La Jolla , CA)
The double mutation K339A , E509A (i.e. a 'knockout' of the charge clamp) was made as a negative control
Cos7 cells were seeded at a density of 1 x 105 cells / cm2 in 12-well plates and transiently transfected with 0.50 痢 of expression vector for ROR alpha wild type or ROR alpha mutants (C288Q and K339A , E509A) together with 1.0 痢 of ROREtkluc and as indicated under 'Materials and Methods.' 24 h after transfection , cells were collected with 250 痞 of passive lysis buffer , and luciferase activity was determined in duplicate on 20 痞 of cell extracts

F.Horn (nucleardbcmbi.kun.nl), 21-Apr-2005